Data Science Seminars: Bioinformatics focus

Speaker:  Carlo Cipriani

January 13t^h, 2026 | 2:15 pm
DEIB, Seminar Room "A. Alario" (Bld. 21)

For further information please contact:  Silvia Cascianelli |  silvia.cascianelli@polimi.it

Abstract

Tuesday, January 13^th, 2026 at 2:15 pm a new appointment of Data Science Seminars: Bioinformatics focus will take place in DEIB "Alessandra Alario" Seminar Room (Building 21) organized by the Data Science for Bioinformatics group.

The seminar will be held by Carlo Cipriani on the following subject: "RAAVioli: A Comprehensive Approach to Characterizing AAV Vector Integrations and Rearrangements".

In this study, we present RAAVioli, a computational method for the comprehensive analysis of AAV integration sites and vector rearrangements across both long- and short-read sequencing platforms. Through in silico benchmarking and in vivo validation in a xenogeneic human hepatocyte model transduced with AAV, we demonstrate the robustness and versatility of RAAVioli in accurately identifying AAV–genome junctions and reconstructing complex vector rearrangements across diverse experimental workflows.
RAAVioli can be applied across different contexts, ranging from gene addition to gene editing. It has already been used to characterize vector integrations in a mouse model of Wilson disease, to study integrations detected in cell-free DNA in patients treated with AAV vectors and from non-human primates across multiple gene-therapy settings, and enabling non-invasive safety and efficacy monitoring.